3D structure of Valkerase enzyme

Published Research: Microbiology and Enzymology

The following is a representative list of research articles written by BRI scientists and published in internationally recognized, peer-reviewed technical journals.

Author(s) Date Name of Article Name of Publication
Shih, J.C.H. 2012 From biogas energy, feed enzyme to new agriculture World Poultry Science Journal, 68:409-416. Erratum: 68:803-806


Shih, J.C.H. and Wang, JJ. 2008 From biogas energy to keratinase technology Biocatalysis and Bioenergy, C.T. Hou and J.K. Shaw (eds.), John Wiley & Son, Inc., Hoboken, New Jersey; Pp. 231-238 in


Chen, C.Y., Rojanatavorn, K., Clark, C. and Shih, J.C.H. 2005 Characterization and enzymatic degradation of Sup35NM, a prion-like protein from yeast. Protein Sci. 14:2228-2235.





Wang J.J., R. Borwornpinyo, N. Odetallah, and J.C.H. Shih 2005 Enzymatic degradation of a prion-like protein, Sup35NM-His6. Enzyme and Microbial Technology 36: 758–765 (SCI)
Wang, J.J., Rojanatavorn, K. and Shih, J. C.H


Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene Biotech. Bioeng. 87:459-464
Langeveld, J.P.M., Wang, J.J, Shih, G.C., Bossers, A., Garssen, J. and Shih, J.C.H. 2003 Enzymatic inactivation of prion protein in brain stem from infected cattle and sheep J. Inf Dis. 188:1782-1789
Wang, J.J. and Shih, J.C.H. 1999 Fermentation Production of Keratinase from Bacillus licheniformis PWD-1 and Recombinant B. subtilis FDB-29 J. Ind. Microb. Biotech. 22:608-616
Lin, X., Kelemen, D. W., Miller, E. S. and Shih, J. C. H. 1995 Nucleotide Sequence and Expression of kerA, the Gene Encoding a Keratinolytic Protease of Bacillus licheniformis PWD- 1. Appl. Env. Microbiol. 61:1469-1474
Lin, X., Lee, C. G., Casale, E. S. and Shih, J. C. H 1992 Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Appl. Environ. Microbiol. 58:3271- 3275
Williams, C. M. and Shih, J. C. H. 1989 Enumeration of Some Microbial Groups in Thermophilic Poultry Waste Digesters and Enrichment of a Feather-Degrading Culture J. Appl. Bacteriol. 67:25-35.
Shih, J. C. H. 1988 Pathogen Control by Anaerobic Digestion Pp. 259-265 in Anaerobic Digestion ‘88. Advance in Water Pollution Control, E. R. Hall and P. N. Hobson (eds), Pergammon Press, Oxford.
Shih, J. C. H. 1987 From Anaerobic Digestion to Holistic Farming Chapter 1, in Bioenvironmental System, Vol. II, D. L. Wise (ed.), p. 1-16, CRC Press, Boca Raton, Florida.
Steinsberger, S. C. and Shih, J. C. H. 1984 The Construction and Operation of a Low-Cost Poultry Waste Digester. Biotech. Bioeng. 26:537- 543
Huang, J. J. and Shih, J. C. H. 1981 The Potential of Biological Methane Generation from Chicken Manure. Biotech. Bioeng. 23:2307-2314